Journal: mSphere

Article Title: Cryptic environmental conjugative plasmid recruits a novel hybrid transposon resulting in a new plasmid with higher dispersion potential

doi: 10.1128/msphere.00252-24

Figure Lengend Snippet: Translocation of Tn7714 into a cryptic conjugative plasmid during conjugation. ( A ) Antibiotic-resistance phenotype of E. coli SW4848 (donor), E. coli DH10B (recipient), and TSW4848-W1 (transconjugant). The antibiotics used were based on the ARGs found in the Tn7714 transposon in panel C. The MICs were repeated three times. ( B ) Whole plasmid gel electrophoresis of E. coli SW4848 and TSW4848-W; this picture was cropped from Fig S1 to show in detail only the large plasmids. E. coli SW4848 also has a small 3 kb plasmid. The sizes of the plasmids were obtained by whole-genome sequencing of E. coli SW4848 and TSW4848-W1. Lane two shows the new IncX plasmid that spontaneously formed by the translocation of the Tn7714 transposon from the IncF plasmid into the new IncX plasmid. ( C ) Description of the relevant plasmids of E. coli SW4848 and TSW4848-W1. The IncF and IncX plasmids belong to SW4848 ( NZ_CP10 7285.1 and NZ_CP107287.1 ), and the new IncX plasmid belongs to TSW4848-W1 ( NZ_CP110861.1 ). All these plasmids have genes coding for conjugation apparatus (red genes). The pink box shows the Tn7714 transposon’s main details. The Δ tnpA gene is a truncated version of the Tn7714 tnpA with an intact inverted repeat right (IRR). The Tn7714 transposon in the IncF plasmid is located between the 54,832 and 77,401 nucleotides and flanked by the 5′-AATTA-3′ DRs. The Tn7714 integrated into the IncX plasmid at the target site 5′-TATGTC-3′ located at nucleotide 5,121. In the new IncX plasmid, the 5′-TATGTC-3′ DRs flank the Tn7714 transposon between the 5,127 and 27,696 nucleotides.

Article Snippet: We used E. coli ATCC 25922 (American Type Culture Collection[ATCC], USA) for quality assurance control ( ).

Techniques: Translocation Assay, Plasmid Preparation, Conjugation Assay, Nucleic Acid Electrophoresis, Sequencing

Journal: mSphere

Article Title: Cryptic environmental conjugative plasmid recruits a novel hybrid transposon resulting in a new plasmid with higher dispersion potential

doi: 10.1128/msphere.00252-24

Figure Lengend Snippet: Experiments to compare the conjugation frequency of IncF and IncX plasmids. ( A ) Depiction of the transformation of E. coli DH10B with the IncF plasmid. We transformed E. coli DH10B with the IncF plasmid to have the IncF plasmid in the same genetic background as the new IncX. We used the transformant SW4848zDH10B to compare the conjugation frequency of the IncF vs the new IncX plasmid. ( B ) Conjugation depiction of E. coli TSW4848-W1 (donor) carrying the new IncX plasmid and E. coli J53 (recipient). ( C ) Conjugation depiction of SW4848zDH10B (donor) carrying the IncF plasmid and E. coli J53 (recipient). ( D ) Antibiotic-resistance phenotype of donors, recipients, and transconjugants depicted in panels B and C. The antibiotics used were based on the ARGs found in the Tn7714 transposon shown in . ( E ) Plasmid phenotype of donors (bacteria with DH10B chromosome) and transconjugants (bacteria with J53 chromosome). Well 1 shows the plasmids of the environmental E. coli SW4848. The plasmid sizes of SW4848 were obtained by whole-genome sequencing. Well 2 shows the new IncX plasmid obtained during the conjugation of E. col i SW4848 (donor) and E. coli DH10B (recipient). The size of the new IncX plasmid (indicated by the red arrow) was obtained after whole-genome sequencing of TSW4848-W1. Well 3 shows that SW4848zDH10B has only the IncF plasmid. Wells 4 and 5 show the successful transfer of the IncF and new IncX plasmids from E. coli DH10B (donor) into E. coli J53 (recipient) during conjugation experiments (this picture was cropped from Fig S1 to show in detail only the large plasmids). ( F ) Conjugation frequency of the IncF and new IncX plasmid from E. coli DH10B (donor) into E. coli J53 (recipient). The plot shows the average conjugation frequency of three independent experiments.

Article Snippet: We used E. coli ATCC 25922 (American Type Culture Collection[ATCC], USA) for quality assurance control ( ).

Techniques: Conjugation Assay, Transformation Assay, Plasmid Preparation, Bacteria, Sequencing

Journal: BioMed Research International

Article Title: Antimicrobial Activity and Chemical Composition of “Kpètè-Kpètè”: A Starter of Benin Traditional Beer Tchoukoutou

doi: 10.1155/2017/6582038

Figure Lengend Snippet: Medium inhibitory diameter of tchoukoutou's starter on reference strains after 24 h and 48 h. E. co 0 : Escherichia coli O157, S. ty : Salmonella typhimurium , Ps. ae : Pseudomonas aeruginos a, S. au : Staphylococcus aureus , S. ep : Staphylococcus epidermidis , Pr. mi : Proteus mirabilis , M. lu : Micrococcus luteus , S. or : Streptococcus oralis , E. co : Escherichia coli , En. fa : Enterococcus faecalis ATCC 29212, Pr. vu : Proteus vulgaris , C. al : Candida albicans .

Article Snippet: shows inhibition diameters of lactic acid bacteria against E. coli ATCC 25922 strain.

Techniques:

Journal: BioMed Research International

Article Title: Antimicrobial Activity and Chemical Composition of “Kpètè-Kpètè”: A Starter of Benin Traditional Beer Tchoukoutou

doi: 10.1155/2017/6582038

Figure Lengend Snippet: Mean inhibition zones' diameter of starter on some isolated pathogenic strains after incubation (24 and 48 h). Tan: Tanguiéta, Nat: Natitingou, Bou: Boukoumbé, Par: Parakou, MRSA: Methicillin Resistant S. aureus , and ESBL EC: extended-spectrum beta-lactamases E. coli. ∗ : p < 0.05.

Article Snippet: shows inhibition diameters of lactic acid bacteria against E. coli ATCC 25922 strain.

Techniques: Inhibition, Isolation, Incubation

Journal: BioMed Research International

Article Title: Antimicrobial Activity and Chemical Composition of “Kpètè-Kpètè”: A Starter of Benin Traditional Beer Tchoukoutou

doi: 10.1155/2017/6582038

Figure Lengend Snippet: Lactic acid bacteria isolates' antimicrobial activity on some pathogenic strains after 24 h and 48 h of incubation. E. co 0: E. coli O157: H7 ATCC 700728, S. ty: Salmonella typhi R 30951401, E. co: E. coli ATCC 25922, S. au: S. aureus ATCC 29213, and c MRSA: clinical isolated MRSA.

Article Snippet: shows inhibition diameters of lactic acid bacteria against E. coli ATCC 25922 strain.

Techniques: Bacteria, Activity Assay, Incubation, Isolation

Journal: BioMed Research International

Article Title: Antimicrobial Activity and Chemical Composition of “Kpètè-Kpètè”: A Starter of Benin Traditional Beer Tchoukoutou

doi: 10.1155/2017/6582038

Figure Lengend Snippet: The inhibitory diameters recorded on bacterial culture after enzymatic hydrolysis.

Article Snippet: shows inhibition diameters of lactic acid bacteria against E. coli ATCC 25922 strain.

Techniques:

Journal: BioMed Research International

Article Title: Antimicrobial Activity and Chemical Composition of “Kpètè-Kpètè”: A Starter of Benin Traditional Beer Tchoukoutou

doi: 10.1155/2017/6582038

Figure Lengend Snippet: Compilation of the tested starters' minimum inhibitory and bactericidal concentration on some references strains.

Article Snippet: shows inhibition diameters of lactic acid bacteria against E. coli ATCC 25922 strain.

Techniques: Concentration Assay, Isolation

Journal: BioMed Research International

Article Title: Antimicrobial Activity and Chemical Composition of “Kpètè-Kpètè”: A Starter of Benin Traditional Beer Tchoukoutou

doi: 10.1155/2017/6582038

Figure Lengend Snippet: Effect of lactic acid bacterial supernatant dilution against E. coli ATCC25922.

Article Snippet: shows inhibition diameters of lactic acid bacteria against E. coli ATCC 25922 strain.

Techniques: Inhibition

Journal: International Journal of Molecular Sciences

Article Title: Current State of Knowledge Regarding WHO High Priority Pathogens—Resistance Mechanisms and Proposed Solutions through Candidates Such as Essential Oils: A Systematic Review

doi: 10.3390/ijms24119727

Figure Lengend Snippet: Studies assessing the antimicrobial activity of essential oils against methicillin resistant, vancomycin-intermediate and -resistant S. aureus (a non-exhaustive list).

Article Snippet: Al-Maharik N et al., 2021 , MRSA S. aureus ATCC 25923 E. faecium ATCC 700221 K. pneumoniae ATCC 13883 Proteus vulgaris ATCC 700221 E. coli ATCC 25922 P. aeruginosa ATCC 27853 C. albicans ATCC 90028 , Satureja nabateorum (Danin and Hedge) Bräuchler , Broth microdilution assay Cell culture cytotoxicity assay , Both the fresh and the air-dried EOs of S. nabateorum presented good and similar antimicrobial and fungicidal activity. EO obtained from the air-dried sample manifested a higher antimicrobial activity against MRSA (MIC = 6.25 μg/mL) than ciprofloxacin (MIC = 12.5 μg/mL). Both EOs showed cytotoxic activity against HeLa and HepG2 cancer cells and were proposed as potential alternatives to bactericides and fungicides of chemical origin, as well as natural preservatives and conservation substances. , [ ] .

Techniques: Activity Assay, Diffusion-based Assay, Inhibition, Cytotoxicity Assay, Electron Microscopy, In Vitro, Dilution Assay, Preserving, Quantitative Proteomics, Nucleic Acid Electrophoresis, Membrane, Confocal Laser Scanning Microscopy, Concentration Assay, Titration, Bacteria, Microscopy, Transmission Assay, Microdilution Assay, Produced, Modification, Clinical Proteomics, Blocking Assay, Staining, Cell Culture, Fourier Transform Infrared Spectroscopy, Spectroscopy, Reverse Transcription, Real-time Polymerase Chain Reaction, Crystal Violet Assay, Expressing, Flow Cytometry, In Vivo, Liposomes, Time-Kill Assay, Formulation, MTT Assay, Incubation, Thin Layer Chromatography, Bioassay, Antibiofilm Assay, Resazurin Assay, Biofilm Production Assay, Control, Infection, Cream, Antioxidant Activity Assay, Permeability, Virus, Extraction, Isolation

Journal: Scientific Reports

Article Title: Extended spectrum betalactamase and carbapenemase producing gram negative bacteria from mobile phones of healthcare workers at Debre Berhan Hospital, Ethiopia

doi: 10.1038/s41598-025-03191-5

Figure Lengend Snippet: Antimicrobial susceptibility pattern of ESBL-positive and ESBL-negative gram-negative bacteria from mobile phones of HCWs at DBCSH from August to October 2022. AMP: ampicillin, AMK: amikacin, CIP: ciprofloxacin, SXT: trimethoprim – sulphamethoxazole C: chloramphenicol, TOB: tobramycin, AUG: augmentin (amoxicillin-clavulanic acid), GM: gentamcin, MRP: meropenem, IMP: imipenem, CTX: cefotaxime, CXM: cefuroxime, CRO: ceftriaxone, TE: tetracycline and CAZ: ceftazidime.

Article Snippet: No zone inhibition of the meropenem susceptible strain ( E. coli ATCC 25922) around the meropenem disk, zone of diameter 6–18 mm or the presence of pinpoint colonies within a 16–18 mm zone of susceptible strain ( E. coli ATCC 25922) around the meropenem disk were considered carbapenemase produce .

Techniques: Bacteria

Journal: Scientific Reports

Article Title: Extended spectrum betalactamase and carbapenemase producing gram negative bacteria from mobile phones of healthcare workers at Debre Berhan Hospital, Ethiopia

doi: 10.1038/s41598-025-03191-5

Figure Lengend Snippet: Antimicrobial susceptibility pattern of Carbapenemase-positive and Carbapenemase-negative gram-negative bacteria from mobile phones of HCWs at DBCSH from August to October 2022. AMP: ampicillin, AMK: amikacin, CIP: ciprofloxacin, SXT: trimethoprim – sulphamethoxazole C: chloramphenicol, TOB: tobramycin, AUG: augmentin (amoxicillin-clavulanic acid), GM: gentamcin, MRP: meropenem, IMP: imipenem, CTX: cefotaxime, CXM: cefuroxime, CRO: ceftriaxone, TE: tetracycline and CAZ: ceftazidime.

Article Snippet: No zone inhibition of the meropenem susceptible strain ( E. coli ATCC 25922) around the meropenem disk, zone of diameter 6–18 mm or the presence of pinpoint colonies within a 16–18 mm zone of susceptible strain ( E. coli ATCC 25922) around the meropenem disk were considered carbapenemase produce .

Techniques: Bacteria

Journal: International Journal of Molecular Sciences

Article Title: Current State of Knowledge Regarding WHO High Priority Pathogens—Resistance Mechanisms and Proposed Solutions through Candidates Such as Essential Oils: A Systematic Review

doi: 10.3390/ijms24119727

Figure Lengend Snippet: Studies assessing the antimicrobial activity of essential oils against methicillin resistant, vancomycin-intermediate and -resistant S. aureus (a non-exhaustive list).

Article Snippet: Leal ALAB et al., 2021 , S. aureus SA1199B (overexpressing nor A gene) S. aureus K2068 (overexpressing mep A gene) S. aureus K4100 (overexpressing qac C gene) C. albicans ATCC 10231 E. coli ATCC 25922 S. aureus ATCC 25923 , Piper caldense C.DC. alone or in combination with norfloxacin , Microdilution , Antimicrobial effect against S. aureus strains was observed only in association with norfloxacin, effect observed at subinhibitory concentrations. In contrast, the EO was active against C. albicans , inhibiting an important mechanism of virulence of the fungi, the hypae formation, therefore having a good antifungal activity. The EO was able to act as efflux pump inhibitor on nor A, mep A and qac C. , [ ] .

Techniques: Activity Assay, Diffusion-based Assay, Inhibition, Cytotoxicity Assay, Electron Microscopy, In Vitro, Dilution Assay, Preserving, Quantitative Proteomics, Nucleic Acid Electrophoresis, Membrane, Confocal Laser Scanning Microscopy, Concentration Assay, Titration, Bacteria, Microscopy, Transmission Assay, Microdilution Assay, Produced, Modification, Clinical Proteomics, Blocking Assay, Staining, Cell Culture, Fourier Transform Infrared Spectroscopy, Spectroscopy, Reverse Transcription, Real-time Polymerase Chain Reaction, Crystal Violet Assay, Expressing, Flow Cytometry, In Vivo, Liposomes, Time-Kill Assay, Formulation, MTT Assay, Incubation, Thin Layer Chromatography, Bioassay, Antibiofilm Assay, Resazurin Assay, Biofilm Production Assay, Control, Infection, Cream, Antioxidant Activity Assay, Permeability, Virus, Extraction, Isolation